Useful Tricks and Scripts ---------------------------- This a list of commonly used scripts for processing of electron microscopy images. Please feel free to share your own. Rafa’s tricks ^^^^^^^^^^^^^^^^ - [[BoxerToXmippMark]] - translate the coordinates of EMAN’s Boxer to Xmipp_Mark - [[CorrectCoordinates]] - correct the coordinates too near to the border of the image - [[CreateMaskFromMap]] - using a binarized, thresholded map - [[RunKerdenSOM]] - apply kerdensom to a set of 2D images or to a set of rotational power spectra - [[SubmitMpiJobOnJumilla]] - run parallel jobs using our Alpha queues. - [[ConvertXmdfileToRelionPosFile]] - After a classfication in Xmipp3, generate Pos file to extract particles in Relion Sjors’ tricks ^^^^^^^^^^^^^^^^ - [[FromXmippToVariance3D]] - use Pawel Penzeck’s Spider scripts after an Xmipp alignment - [[SelectionFileSpidertoXmipp]] - convert Spider selection file (with numbers only) to an Xmipp selfile - [[XmippPreprocessing]] - Tiff2raw, CTF-estimation, extraction, normalization, phase correction etc. - [[MovingParticles]] - analyze the behaviour of your particles in a ML classification experiment. - [[SortImagesBasedOnDocfile]] - Make a sorted selfile from a newxmipp style docfile (e.g. sort by CC). - [[CtffindToXmippCtfParam]] - convert the .ctf file from CTFFIND to an XMIPP CTF-parameter file - [[CompareClassAveragesWithReprojections]] - Align reference-free class averages with projections of a 3D model and visualize Roberto’s tricks ^^^^^^^^^^^^^^^^ - [[OpenDX]] - visualize Xmipp volumes in opendx - [[CompareVolumeT]] using python (Note volumeT is the base clase of [[VolumeXmipp]]) - - - - [[CTFParamOldNew]] Convert ctf from old to new (metadata - [[ExportPos]] - [[CtfFindToXmippThree]] Coss’ tricks ^^^^^^^^^^^^^^^^ - [[WebToXmippMark]] - How to convert coordinates of particles picked with Web (Spider) into a set of particles picked with Xmipp - [[EstimatingNoisePowerForAGivenSNR]] - How to estimate how much noise to add to a set of phantom projections to have a given average SNR - [[CoSSSmallTricks]] - How to generate an empty image, compute a radial average, … - [[EstimateTheCTF]] - How to estimate the CTF - [[RunMpiProgramsWithoutRepeatingThePassword]] - How to run MPI programs without having to repeat the password for each process (depends on the UNIX distribution) - [[CorrectingMisalignmentOfPDB]] - How to look for a misaligned rotational symmetry axis in a PDB and correct it - [[HowManyReferenceProjections]] - What is the number of reference projections given an angular step - [[EquivalentEulerAngles]] - Given an Euler angle, what are the Euler angles of its X, Y and XY mirrors and how are they expressed with another set of angles - [[ConvertImagesToXmipp]] - Given images in EMAN, CCP4, Spider, … How to convert them to Xmipp - [[RemoveOpenMPIWarnings]] - Remove [[OpenMPI]] warning about openib and udapl - [[EulerAngles]] - What is the interpretation of Euler angles in Xmipp - [[HexDumpF]] - Hexdump of float numbers considering endianness - [[ConvertingFrealignXmipp]] - Conversion of Euler angles from FRealign to Xmipp - [[ConvertingLstXmipp]] - Conversion of Eman lst files to Xmipp - [[DefinedMacros]] - How to know the macros defined by a compiler in a given system - [[ConvertDownsamplePos]] - Convert and downsample posfiles of Xmipp 3.0 to Xmipp 2.4 - [[ImportPosFromEmanAndSpider]] - Import coordinates from Eman and Spider into Xmipp 3.0 - [[ApplyGeoInReconstructionMetadata]] - Given a metadata for reconstruction, generate the aligned images needed for reconstructing without shifts and mirrors - [[GenerateTiltPairs]] - Generate untilted and tilted images for a given volume Carmen’s scripts ^^^^^^^^^^^^^^^^ - If you have performed a projection matching after downsampling the micrographs by a certain factor, and now want to proceed with a different downsampling factor (eg to use a finer pixel size), you may find the next two shell scripts useful: - [[ScalePos]] - to write new .pos files according to the new downsampling (so you do not have to pick particles again) - [[ScaleShifts]] - to reuse angles and shifts found in the previous projection matching run